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KMID : 0043320180410050490
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Archives of Pharmacal Research
2018 Volume.41 No. 5 p.490 ~ p.496
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Tyrosinase inhibitory components from the seeds of Cassia tora
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Lee Ga-Young
Cho Byoung-Ok
Shin Jae-Young
Jang Seon-Il
Cho In-Sook
Kim Hyo-Young
Park Ji-Su
Cho Chong-Woon
Kang Jong-Seong
Kim Jang-Hoon
Kim Young-Ho
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Abstract
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Ten compounds (1?10) isolated from the seeds of Cassia tora were evaluated for tyrosinase inhibition. Compounds 3, 4, and 7 inhibited tyrosinase enzymatic activity in a dose-dependent manner, with IC50 values of 3.0?±?0.8, 7.0?±?0.4, and 9.2?±?3.4 μM, respectively. Kinetic analyses revealed a mechanism consistent with competitive inhibition. In silico molecular docking showed that compounds 3 and 4 docked in the active site of tyrosinase, whereas 7 interacted with Ala246 and Val248 at outside of the active site, and His244 and Glu256 at inside. Additionally, compounds 3, 4, and 7 suppressed melanogenesis in α-MSH-treated B16F10 melanoma cells at a concentration of 10 μM.
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KEYWORD
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Cassia tora, Tyrosinase inhibitor, Competitive type, Molecular docking, Melanogenesis
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